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KMID : 0376219840210030449
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Chonnam Medical Journal
1984 Volume.21 No. 3 p.449 ~ p.457
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Electron Microscopic Study of Hepatocyte in Rat Fed a High Cholesterol Diet
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Abstract
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To investigate the effect of a high cholesterol diet on the rat hepatocyte, electron microscopic observations were made. Rats were fed a atherogenic diet containing high cholesterol-cholic acid for five and ten weeks, and sacrificed 24 hours after last administration of the diet. Results obtained were summerized as follows.
In the rats fed the diet for five weeks, hepatocytes contained variable amount of lipid droplets. These lipid droplets were seen in the cytoplasm, nucleus and Golgi vacuoles. Perisinusoidal spaces appeared to have considerable amount of collagen fibers.
In the rats fed the diet for ten weeks, fat changes became more pronounced than those in five weeks feeding group. Intranuclear lipid droplets increased in number and were considered to be true inclusions. In addition, some cytoplasmic changes became apparent. Proliferation of smooth endoplasmic reticulum concomitant with a depletion of glycogen was noted. Bile canaliculi often showed dilatation, and contained fragmented or myelinated materials.
These results indicate that high cholesterol diet leads to the fat accumulation in the cytoplasm and nucleus of the hepatocytes, and that the number of nuclear fat inclusions is closely related with the duration of the high cholesterol diet.
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KEYWORD
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